The Crew plays 10 reserve matches this year, kicking off with a First Kick doubleheader against the Montreal Impact on March 24th. The reserves also play the Red Bulls, Revolution, D.C. United, and the Union at home. They also play Toronto, the Revs, D.C., the Union, and Red Bulls on the road. Full schedule follows.
| Date | Home Team | Away Team | Time | Location |
| 3/24/2012 | Columbus Crew | Montreal Impact | 6:30:00 PM | Crew Stadium |
| 3/31/2012 | Toronto FC | Columbus Crew | 4:30:00 PM | BMO Field |
| 4/7/2012 | Columbus Crew | NY Red Bulls | 5:00:00 PM | Crew Stadium |
| 4/15/2012 | Philadelphia Union | Columbus Crew | 6:00:00 PM | Chester Park |
| 6/10/2012 | Columbus Crew | NE Revolution | 10:00:00 AM | Crew Stadium |
| 6/17/2012 | NE Revolution | Columbus Crew | 11:00:00 AM | Gillette Stadium |
| 7/22/2012 | Columbus Crew | D.C. United | 11:00:00 AM | Crew Stadium |
| 8/5/2012 | D.C. United | Columbus Crew | 11:00:00 AM | DCU training field |
| 9/16/2012 | New York Red Bulls | Columbus Crew | 11:00:00 AM | Montclair St. University |
| 9/30/2012 | Columbus Crew | Philadelphia Union | 11:00:00 AM | Crew Stadium |